Tomato hybrid T316108

ABSTRACT

The invention provides tomato hybrid T316108 or line A11D13TEN000015 and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a tomato plant by crossing the tomato plants of the invention with themselves or another tomato plant. The invention also provides tomato plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom.

FIELD OF THE INVENTION

This invention is in the field of tomato plants.

BACKGROUND OF THE INVENTION

Tomato is an important global crop. Several plant species associatedwith the family Solanaceae have been familiar to mankind since ancienttimes and are of great agricultural importance. The genus Solanum is amember of the Solanaceae family and includes the domesticated tomato S.lycopersicum (also known as Lycopersicum esculentum). Tomato isgenerally adapted to warm summer growing conditions but can also begrown in heated greenhouses under winter conditions. The introduction ofhybrid tomato cultivars in the 1950s provided a magnitude of benefitsincluding increased yield, better holding ability, adaptation toexpanded growing seasons through the use of protected cultivation andimproved disease resistances, which resulted in large-scale productionof tomato as a commercial crop.

Tomato breeders make continual improvements in hybrid tomato yield,horticultural characteristics, and consumer quality traits. Thus, thereis an ongoing need for improved tomato hybrid varieties and parentallines.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel hybrid tomatovariety, designated Monile, also known as T316108, and parental tomatoline AI1D13TEN000015.

The invention also encompasses the seeds of tomato hybrid T316108 andline AI1D13TEN000015, the plants of tomato hybrid T316108 and lineAI1D13TEN000015, plant parts of the tomato hybrid T316108 and lineAI1D13TEN000015 (including fruit, seed, gametes, rootstock, scions,shoots), methods of producing seed from tomato hybrid T316108 and lineAI1D13TEN000015, and methods for producing a tomato plant by crossingthe tomato hybrid T316108 or line AI1D13TEN000015 with itself or anothertomato plant, methods for producing a tomato plant containing in itsgenetic material one or more transgenes, and the transgenic tomatoplants produced by that method. The invention also relates to methodsfor producing other tomato plants derived from tomato hybrid T316108 orline AI1D13TEN000015 and to tomato plants, parts thereof and seedproduced by the use of those methods. The present invention furtherrelates to hybrid tomato seeds and plants (and parts thereof includingfruit) produced by crossing tomato hybrid T316108 or lineAI1D13TEN000015 with another tomato plant.

The invention further contemplates grafted tomato plants and methods ofproducing a grafted tomato plant, where tomato hybrid T316108 or lineAI1D13TEN000015 can be used as either the rootstock or the scion.

In another aspect, the present invention provides regenerable cells foruse in tissue culture of tomato hybrid T316108 and line AI1D13TEN000015.In embodiments, the tissue culture is capable of regenerating plantshaving all or essentially all of the physiological and morphologicalcharacteristics of the foregoing tomato plant and/or of regeneratingplants having the same or substantially the same genotype as theforegoing tomato plant. In embodiments, the regenerated plant is adiploid plant. In exemplary embodiments, the regenerable cells in suchtissue cultures are meristematic cells, cotyledons, hypocotyl, leaves,pollen, embryos, roots, root tips, anthers, pistils, ovules, shoots,stems, petiole, pith, flowers, capsules and/or seeds as well as callusand/or protoplasts derived from any of the foregoing. Still further, thepresent invention provides tomato plants regenerated from the tissuecultures of the invention.

As a further aspect, the invention provides a method of producing tomatoseed, the method comprising crossing a plant of tomato hybrid T316108 orline AI1D13TEN000015 with itself or a second tomato plant, cultivatingthe plant to maturity, and allowing seed to form. Optionally, the methodfurther comprises collecting the seed.

Another aspect of the invention provides methods for producing hybridsand other tomato plants derived from tomato hybrid T316108 or lineAI1D13TEN000015. Tomato plants derived by the use of these methods arealso part of the invention as well as plant parts, seed, gametes andtissue culture from such hybrid or derived tomato plants.

In representative embodiments, a tomato plant derived from tomato hybridT316108 or line AI1D13TEN000015 comprises cells comprising at least oneset of chromosomes derived from tomato hybrid T316108 or lineAI1D13TEN000015. Those skilled in the art will appreciate that a plantderived from hybrid T316108 is also derived from parental lineAI1D13TEN000015.

In embodiments, a tomato plant or population of tomato plants derivedfrom tomato hybrid T316108 or line AI1D13TEN000015 comprises, onaverage, at least 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%,65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of its alleles (i.e.,theoretical allelic content; TAC) from tomato hybrid T316108 or lineAI1D13TEN000015, e.g., at least about 6.25%, 12.5%, 25%, 30%, 35%, 40%,45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99%of the genetic complement of tomato hybrid T316108 or lineAI1D13TEN000015, respectively, and optionally may be the result of abreeding process comprising one or two breeding crosses and one or moreof selfing, sibbing, backcrossing and/or double haploid techniques inany combination and any order. In embodiments, the breeding process doesnot include a breeding cross, and comprises selfing, sibbing,backcrossing and or double haploid technology. In embodiments, thetomato plant derived from tomato hybrid T316108 or line AI1D13TEN000015is one, two, three, four, five or more breeding crosses removed fromtomato hybrid T316108 or line AI1D13TEN000015, respectively.

In embodiments, a hybrid or derived plant from tomato hybrid T316108 orline AI1D13TEN000015 comprises a desired added trait(s). Inrepresentative embodiments, a tomato plant derived from tomato hybridT316108 or line AI1D13TEN000015 comprises all of the morphological andphysiological characteristics of tomato hybrid T316108 or lineAI1D13TEN000015 (e.g., as described in Table 1), respectively, with theaddition of the desired added trait(s). In embodiments, the tomato plantderived from tomato hybrid T316108 or line AI1D13TEN000015 comprisesessentially all of the morphological and physiological characteristicsof tomato hybrid T316108 or line AI1D13TEN000015 (e.g., as described inTable 1), respectively, with the addition of a desired added trait(s).

The invention also relates to methods for producing a tomato plantcomprising in its genetic material one or more transgenes and to thetransgenic tomato plant produced by those methods (and progeny tomatoplants comprising the transgene). Also provided are plant parts, seedand tissue culture from such transgenic tomato plants, optionallywherein one or more cells in the plant part, seed, or tissue culturecomprises the transgene. The transgene can be introduced via planttransformation and/or breeding techniques (e.g., once planttransformation of a precursor plant has been achieved, it can then betransferred to other plants via conventional breeding).

In another aspect, the present invention provides for single locusconverted plants of tomato hybrid T316108 and line AI1D13TEN000015.Plant parts, seed, and tissue culture from such single locus convertedplants are also contemplated by the present invention. The single locusmay be a dominant or recessive allele. The single locus may be anaturally occurring tomato locus (e.g., introduced via traditionalbreeding or gene editing techniques), a transgene introduced into tomatothrough genetic engineering techniques (of the plant or a precursorthereof), or a non-naturally occurring locus (e.g., produced bymutagenesis or gene editing).

The invention further provides methods for developing tomato plants in atomato plant breeding program using plant breeding techniques including,for example, recurrent selection, backcrossing, pedigree breeding,double haploid techniques, restriction fragment length polymorphismenhanced selection, genetic marker enhanced selection, gene editingand/or genetic transformation. Seeds, tomato plants, and parts thereof,produced by such breeding methods are also part of the invention.

The invention also provides methods of multiplication or propagation oftomato plants of the invention, which can be accomplished using anymethod known in the art, for example, via vegetative propagation and/orseed.

Additional aspects of the invention include harvested products andprocessed products from the tomato plants of the invention. A harvestedproduct can be a whole plant or any plant part, as described herein.Thus, in some embodiments, a non-limiting example of a harvested productincludes a seed, a fruit (e.g., including the flesh), a rootstock, ascion and/or a shoot.

In representative embodiments, a processed product includes, but is notlimited to: cut, sliced, ground, dried, pureed, canned, jarred, washed,packaged, frozen, seeded, peeled and/or heated fruit or fruit flesh ofthe tomato plants of the invention, or any other part thereof, includingpuree, paste, sauce and salsa made from fruits or fruit flesh.

The seed of the invention can optionally be provided as an essentiallyhomogenous population of seed of a single plant or cultivar. Essentiallyhomogenous populations of seed are generally free from substantialnumbers of other seed, e.g., at least about 90%, 95%, 96%, 97%, 98% or99% pure.

In representative embodiments, the invention provides a seed of tomatohybrid T316108 or line AI1D13TEN000015.

As a further aspect, the invention provides a plant of tomato hybridT316108 or line AI1D13TEN000015.

As an additional aspect, the invention provides a tomato plant, or apart thereof, having all or essentially all of the physiological andmorphological characteristics of a plant of tomato hybrid T316108 orline AI1D13TEN000015.

As another aspect, the invention provides fruit and fruit flesh of thetomato plants of the invention and processed products from the fruit orfruit flesh of the inventive tomato plants.

As still another aspect, the invention provides a method of producingtomato seed, the method comprising crossing a tomato plant of theinvention with itself or a second tomato plant (e.g., crossing lineAI1D13TEN000015 with another tomato line to produce a F1 hybrid seed(e.g., seed of T316108). In embodiments, the method is practiced toproduce seed of line AI1D13TEN000015 (e.g., seed increase). Theinvention also provides seed produced by this method and plants, andparts thereof including fruit, produced by growing the seed.

As yet a further aspect, the invention provides a method for producing aseed of a tomato plant derived from tomato hybrid T316108 or lineAI1D13TEN000015, the method comprising: (a) crossing a tomato plant oftomato hybrid T316108 or line AI1D13TEN000015 with a second tomatoplant; and (b) allowing seed of a tomato plant derived from tomatohybrid T316108 or line AI1D13TEN000015 to form. In embodiments, themethod further comprises: (c) growing a plant from the seed derived fromtomato hybrid T316108 or line AI1D13TEN000015 of step (b); (d) selfingthe plant of step (c) or crossing it to a second tomato plant to formadditional tomato seed derived from tomato hybrid T316108 or lineAI1D13TEN000015, and (e) optionally repeating steps (c) and (d) one ormore times to generate further derived tomato seed from tomato hybridT316108 or line AI1D13TEN000015, wherein in step (c) a plant is grownfrom the additional tomato seed of step (d) in place of growing a plantfrom the seed of step (b). In embodiments, the method comprises: (e)repeating steps (c) and (d) one or more times (e.g., three or more, fouror more, five or more, six or more, one to three, one to five, one tosix, one to seven, one to ten, three to five, three to six, three toseven, three to eight or three to ten times) to generate further derivedtomato seed. As another option, the method can comprise cultivating theplant to maturity and collecting the seed. The invention also providesseed produced by these methods and tomato plants produced by growing theseed.

Still further, as another aspect, the invention provides a method ofvegetatively propagating a plant of tomato hybrid T316108 or lineAI1D13TEN000015, e.g., via shoot proliferation and then rooting intissue culture. In a non-limiting example, the method comprises: (a)collecting tissue capable of being propagated from a plant of tomatohybrid T316108 or line AI1D13TEN000015; (b) cultivating the tissue toobtain proliferated shoots; and (c) rooting the proliferated shoots toobtain rooted plantlets. Optionally, the invention further comprisesgrowing plants from the rooted plantlets. The invention also encompassesthe plantlets and plants produced by these methods, as well as seed andfruits produced therefrom.

As a further aspect, the invention provides a method of producing agrafted tomato plant, wherein hybrid T316108 or line AI1D13TEN000015 isused as either the rootstock or the scion.

As an additional aspect, the invention provides a method of introducinga desired added trait into tomato hybrid T316108 or lineAI1D13TEN000015, the method comprising: (a) crossing a first plant oftomato hybrid T316108 or line AI1D13TEN000015 with a second tomato plantthat comprises a desired added trait to produce F₁ progeny; (b)selecting an F₁ progeny that comprises the desired trait; (c) crossingthe selected F₁ progeny with tomato hybrid T316108 or lineAI1D13TEN000015 to produce backcross progeny; and (d) selectingbackcross progeny comprising the desired trait to produce a plantderived from tomato hybrid T316108 or line AI1D13TEN000015 comprising adesired trait.

In embodiments, the selected backcross progeny when tomato hybridT316108 is used as the recurrent parent is characterized by 1, 2, 3, 4or more of the characteristics of tomato hybrid T316108 as describedherein, e.g., produces a strong generative plant type with goodconsistent setting, mature fruit with a shiny pink color and flesh, asmall uniform fruit size, an oval plum shape, and/or an excellent taste.In embodiments, the selected backcross progeny when tomato lineAI1D13TEN000015 is used as the recurrent parent is characterized by 1,2, 3, 4 or more of the characteristics of tomato line AI1D13TEN000015 asdescribed herein, e.g., produces a mature fruit with a yellow skincolor, a uniform yellow mature fruit flesh and/or an oval plum shape. Inembodiments, the selected progeny comprises all or essentially all themorphological and physiological characteristics of tomato hybrid T316108or line AI1D13TEN000015 (e.g., a described in Table 1).

Optionally, the method further comprises: (e) repeating steps (c) and(d) one or more times (e.g., one to three, one to five, one to six, oneto seven, one to ten, three to five, three to six, three to seven, threeto eight or three to ten times) to produce a plant derived from tomatohybrid T316108 or line AI1D13TEN000015 comprising the desired trait,wherein in step (c) the selected backcross progeny produced in step (d)is used in place of the selected F1 progeny of step (b). In embodiments,the backcross of step (c) is repeated at least three, four, five, six ormore times to produce an inbred line derived from tomato hybrid T316108or line AI1D13TEN000015 and, optionally, the method further comprisescrossing the derived inbred line with a different tomato line to producean F1 hybrid progeny tomato plant derived from tomato hybrid T316108 orline AI1D13TEN000015.

In representative embodiments, the invention also provides a method ofproducing a plant of tomato hybrid T316108 or line AI1D13TEN000015comprising a desired added trait, the method comprising introducing atransgene conferring the desired trait into a plant of tomato hybridT316108 or line AI1D13TEN000015. The transgene can be introduced bytransformation methods (e.g., genetic engineering) or traditionalbreeding techniques (e.g., once the transgene is introduced into aprecursor plant).

The invention also provides tomato plants produced by the methods of theinvention, wherein the tomato plant has the desired added trait as wellas seed and fruits from such tomato plants. The invention also providesseed that produces the plants derived from hybrid T316108 or lineAI1D13TEN000015 and comprising a desired added trait.

According to the foregoing methods, the desired added trait can be anysuitable trait known in the art including without limitation: malesterility, male fertility, herbicide resistance, pest (e.g., insectand/or nematode) resistance, modified fatty acid metabolism, modifiedcarbohydrate metabolism, disease resistance (e.g., against bacterial,fungal and/or viral disease), abiotic stress tolerance, enhancednutritional quality (e.g., increased flavonoid content of the fruit),improved appearance (e.g., external fruit color and/or fruit fleshcolor), increased fruit sweetness, increased fruit flavor, improvedfruit ripening, improved texture or taste, improved fruit yield,improved seed yield, improved seedling vigor, improved seed germination,industrial usage, or any combination thereof.

In representative embodiments, a transgene conferring herbicideresistance confers resistance to glyphosate, sulfonylurea,imidazolinone, dicamba, glufosinate, phenoxy proprionic acid,L-phosphinothricin, cyclohexone, cyclohexanedione, triazine,benzonitrile, or any combination thereof.

In representative embodiments, a transgene conferring pest resistance(e.g., insect and/or nematode resistance) encodes a Bacillusthuringiensis endotoxin.

In representative embodiments, transgenic plants, transformed plants(e.g., using genetic engineering techniques), gene edited plants, singlelocus converted plants, hybrid plants and tomato plants derived fromtomato hybrid T316108 are characterized by 1, 2, 3, 4 or more of thecharacteristics of tomato hybrid T316108 as described herein, e.g.,produces a strong generative plant type with good consistent setting,mature fruit with a shiny pink color and flesh, a small uniform fruitsize, an oval plum shape, and/or an excellent taste. In embodiments,transgenic plants, transformed plants (e.g., using genetic engineeringtechniques), gene edited plants, single locus converted plants, hybridplants and tomato plants derived from tomato hybrid T316108 from tomatoline AI1D13TEN000015 are characterized by 1, 2, 3, 4 or more of thecharacteristics of tomato line AI1D13TEN000015 as described herein,e.g., produces a mature fruit with a yellow skin color, a uniform yellowmature fruit flesh and/or an oval plum shape. In representativeembodiments, transgenic plants, transformed plants, gene edited plants,hybrid plants and tomato plants derived from tomato hybrid T316108 orline AI1D13TEN000015 comprise essentially all of the morphological andphysiological characteristics of tomato hybrid T316108 or lineAI1D13TEN000015 (for example, as described in Table 1), respectively, oreven all of the morphological and physiological characteristics oftomato hybrid T316108 or line AI1D13TEN000015, so that said plants arenot significantly different for said traits than tomato hybrid T316108or line AI1D13TEN000015, respectively, as determined at the 5%significance level when grown in the same environmental conditions;optionally, with the presence of one or more desired additional traits(e.g., as described above).

In one embodiment, the present invention discloses a method of producingseeds of tomato hybrid T316108 or line AI1D13TEN000015 comprisinggrowing a plant of tomato hybrid T316108 or line AI1D13TEN000015;allowing pollination of said plants, for example, open-pollination ofsaid plants in an isolated plot or field; and harvesting seeds from saidplants. In one embodiment, the method further comprises washing anddrying said seed.

The invention further provides a method of developing a tomato variety(e.g., a diploid tomato variety) in a tomato plant breeding programusing plant breeding techniques, which include employing a tomato plant,or a part thereof, as a source of plant breeding material, the methodcomprising: (a) obtaining the tomato plant, or a part thereof, of hybridT316108 or line AI1D13TEN000015 as a source of breeding material; and(b) applying plant breeding techniques.

The invention also encompasses plant parts, plant material, pollen,ovules, leaves, fruit and seed from the tomato plants of the invention.The invention also provides seed that produces the tomato plants of theinvention. Also provided is a tissue culture of regenerable cells fromthe tomato plants of the invention, where optionally, the regenerablecells are: (a) embryos, meristem, leaves, pollen, cotyledons,hypocotyls, roots, root tips, anthers, flowers, pistils, ovules, seed,shoots, stems, stalks, petioles, pith and/or capsules; or (b) callus orprotoplasts derived from the cells of (a). Further provided are tomatoplants regenerated from a tissue culture of the invention.

In still yet another aspect, the invention provides a method ofdetermining a genetic characteristic of tomato hybrid T316108 or lineAI1D13TEN000015 or a progeny thereof, e.g., a method of determining agenotype of tomato hybrid T316108 or line AI1D13TEN000015 or a progenythereof using molecular genetic techniques. In embodiments, the methodcomprises detecting in the genome of a tomato hybrid T316108 or lineAI1D13TEN000015 plant, or a progeny plant thereof, at least a firstpolymorphism, e.g., comprises nucleic acid amplification and/or nucleicacid sequencing. To illustrate, in embodiments, the method comprisesobtaining a sample of nucleic acids from the plant and detecting atleast a first polymorphism in the nucleic acid sample (e.g., using oneor more molecular markers). Optionally, the method may comprisedetecting a plurality of polymorphisms (e.g., two or more, three ormore, four or more, five or more, six or more, eight or more or ten ormore polymorphisms, etc.) in the genome of the plant. In representativeembodiments, the method further comprises storing the results of thestep of detecting the polymorphism(s) on a computer readable medium. Theinvention further provides a computer readable medium produced by such amethod.

In addition to the exemplary aspects and embodiments described above,the invention is described in more detail in the description of theinvention set forth below.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is based, in part, on the development of a noveltomato hybrid T316108 and parental line AI1D13TEN000015. Among othercharacteristics, when grown under standard horticultural conditions(e.g., greenhouse), tomato hybrid T316108 is characterized by a maturefruit having a shiny pink skin color, a pink mature fruit flesh, ovalplum shape, an average weight of about 10 grams, and an excellent taste.Among other characteristics, when grown under standard horticulturalconditions (e.g., greenhouse), tomato line AI1D13TEN000015 ischaracterized by a mature fruit having a yellow skin color, a uniformyellow fruit flesh, oval plum shape, an average weight of about 9 to 10grams, and an average diameter of about 2.8 cm.

It should be appreciated that the invention can be embodied in differentforms and should not be construed as limited to the embodiments setforth herein. Rather, these embodiments are provided so that thisdisclosure will be thorough and complete, and will fully convey thescope of the invention to those skilled in the art.

Unless the context indicates otherwise, it is specifically intended thatthe various features and embodiments of the invention described hereincan be used in any combination.

Moreover, the present invention also contemplates that in someembodiments of the invention, any feature or combination of features setforth herein can be excluded or omitted. To illustrate, if thespecification states that a composition comprises components A, B and C,it is specifically intended that any of A, B or C, or a combinationthereof, can be omitted and disclaimed singularly or in any combination.

Unless otherwise defined, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which this invention belongs. The terminology used in thedescription of the invention herein is for the purpose of describingparticular embodiments only and is not intended to be limiting of theinvention.

All publications, patent applications, patents, and other referencesmentioned herein are incorporated by reference in their entirety.

Definitions.

In the description and tables that follow, a number of terms are used.In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided:

As used in the description of the invention and the appended claims, thesingular forms “a,” “an” and “the” are intended to include the pluralforms as well, unless the context clearly indicates otherwise.

As used herein, “and/or” refers to and encompasses any and all possiblecombinations of one or more of the associated listed items, as well asthe lack of combinations when interpreted in the alternative (“or”).

The term “about,” as used herein when referring to a measurable valuesuch as a dosage or time period and the like, is meant to encompassvariations of ±20%, ±10%, ±5%, ±1%, ±0.5%, or even ±0.1% of thespecified amount.

The term “comprise,” “comprises” and “comprising” as used herein,specify the presence of the stated features, integers, steps,operations, elements, and/or components, but do not preclude thepresence or addition of one or more other features, integers, steps,operations, elements, components, and/or groups thereof.

As used herein, the transitional phrase “consisting essentially of”means that the scope of a claim is to be interpreted to encompass thespecified materials or steps recited in the claim “and those that do notmaterially affect the basic and novel characteristic(s)” of the claimedinvention. See, In re Herz, 537 F.2d 549, 551-52, 190 U.S.P.Q. 461, 463(CCPA 1976) (emphasis in the original); see also MPEP § 2111.03. Thus,the term “consisting essentially of” when used in a claim or thedescription of this invention is not intended to be interpreted to beequivalent to “comprising.”

“Added trait”: As used herein, a desired “added trait” or “additionaltrait” can be any trait that confers a desired characteristic to theplant, and may be introduced by any method known in the art, e.g.,conventional breeding (for example, backcrossing), gene editing,mutagenesis, or genetic transformation techniques. Examples of addedtraits include without limitation: male sterility, male fertility,herbicide resistance, pest (e.g., insect and/or nematode) resistance,modified fatty acid metabolism, modified carbohydrate metabolism,disease resistance (e.g., against bacterial, fungal and/or viraldisease), abiotic stress tolerance, enhanced nutritional quality (e.g.,increased flavonoid content of the fruit), improved appearance (e.g.,external fruit color and/or fruit flesh color), increased fruitsweetness, increased fruit flavor, improved fruit ripening, improvedtexture or taste, improved fruit yield, improved seed yield, improvedseedling vigor, improved seed germination, industrial usage, geneediting machinery, or any combination thereof. The trait can be encodedby a DNA sequence (e.g., native tomato sequence, induced or naturallyoccurring mutation, edited native sequence, or a transgene) or canresult from expression of a functional non-translated RNA (e.g., RNAinterference).

“Allele”. An allele is any of one or more alternative forms of a gene,all of which relate to a trait or characteristic. In a diploid cell ororganism, the two alleles of a given gene occupy the corresponding locuson a pair of homologous chromosomes.

“Backcrossing”. Backcrossing is a process in which a breeder repeatedlycrosses hybrid progeny back to one of the parents (the “recurrent”parent), for example, a first generation hybrid F₁ with one of theparental genotype of the F₁ hybrid.

“Cotyledon”. One of the first leaves of the embryo of a seed plant;typically one or more in monocotyledons, two in dicotyledons, and two ormore in gymnosperms.

“Determinate”: Determinate tomato plants tend to be compact and bushy inform, and the plant produces all of its fruit simultaneously within ashort period of time (e.g., one to three weeks), and then fruitproduction stops.

“Double haploid line”. A stable inbred line achieved by doubling thechromosomes of a haploid line, e.g., from anther culture. For example,some pollen grains (haploid) cultivated under specific conditionsdevelop plantlets containing 1n chromosomes. The chromosomes in theseplantlets are then induced to “double” (e.g., using chemical means)resulting in cells containing 2n chromosomes. The progeny of theseplantlets are termed “double haploid” and are essentially notsegregating any more (e.g., are stable). The term “double haploid” isused interchangeably herein with “dihaploid.”

“Essentially all of the physiological and morphologicalcharacteristics”. A plant having “essentially all the physiological andmorphological characteristics” (and similar phrases) means a planthaving all of the desired physiological and morphologicalcharacteristics of hybrid T316108 or line AI1D13TEN000015, except forthe characteristic(s) derived from a converted locus/loci (e.g., asingle converted locus), for example, introduced via backcrossing tohybrid T316108 or line AI1D13TEN000015, a modified gene(s) resultingfrom genome editing techniques, an introduced transgene (i.e.,introduced via genetic transformation techniques) or mutation, when bothplants are grown under the same environmental conditions. Inembodiments, a plant having “essentially all of the physiological andmorphological characteristics” means a plant having all of thecharacteristics of the reference plant with the exception of five orfewer traits, 4 or fewer traits, 3 or fewer traits, 2 or fewer traits,or one trait. In embodiments, the plant comprising “essentially all ofthe physiological and morphological characteristics” of variety (e.g.,for tomato hybrid T316108, a strong generative plant type with goodconsistent setting, mature fruit with a shiny pink color and flesh, asmall uniform fruit size, and/or an oval plum shape or any other traitdescribed herein, e.g., in Table 1; and for tomato line AI1D13TEN000015,a mature fruit with a yellow skin color, a uniform yellow mature fruitflesh and/or an oval plum shape or any other trait described herein,e.g., in Table 1).

“First water date”. The date the seed first receives adequate moistureto germinate. This can and often does equal the planting date.

“Gene”. As used herein, “gene” refers to a segment of nucleic acidcomprising an open reading frame. A gene can be introduced into a genomeof a species, whether from a different species or from the same species,using transformation or various breeding methods.

“Gene editing”: “Gene editing” (sometimes also called “genome editing”)refers to the introduction of targeted modifications into genomic DNAusing techniques employing, for example, meganucleases, zinc-fingernucleases, TALENs, and CRISPR/Cas9 systems. See, e.g., Gaj et al., ZFN,TALEN, and CRISPR/Cas-based methods for genome engineering. Trends inBiotechnology, 31:397-405 (2013).

“Inbred line”: As used herein, the phrase “inbred line” refers to agenetically homozygous or nearly homozygous population. An inbred line,for example, can be derived through several cycles of sib crossingand/or selfing and/or via double haploid production. In someembodiments, inbred lines breed true for one or more traits of interest.An “inbred plant” or “inbred progeny” is an individual sampled from aninbred line.

“Indeterminate”: Indeterminate tomato plants tend to be vine-like, andwill grow and produce fruits throughout the season (e.g., until killedby frost).

“Plant.” As used herein, the term “plant” includes plant cells, plantprotoplasts, plant cell tissue cultures from which plants can beregenerated, plant calli, plant clumps, and plant cells that are intactin plants or parts of plants, such as leaves, pollen, embryos,cotyledons, hypocotyl, roots, root tips, anthers, pistils, flowers,ovules, seeds, fruit, stems, and the like.

“Plant material”. The terms “plant material” and “material obtainablefrom a plant” are used interchangeably herein and refer to any plantmaterial obtainable from a plant including without limitation, leaves,stems, roots, flowers or flower parts, fruits, pollen, ovules, zygotes,seeds, cuttings, cell or tissue cultures, or any other part or productof the plant.

“Plant part”. As used herein, a “plant part” includes any part, organ,tissue or cell of a plant including without limitation an embryo,meristem, leaf, pollen, cotyledon, hypocotyl, root, root tip, anther,flower, flower bud, pistil, ovule, seed, shoot, stem, stalk, petiole,pith, capsule, a scion, a rootstock and/or a fruit including callus andprotoplasts derived from any of the foregoing. In representativeembodiments, the plant part is a non-propagating plant part, forexample, is not a seed.

“Quantitative Trait Locus”. Quantitative Trait Locus (QTL) refers to agenetic locus that control to some degree, numerically representabletraits that are usually continuously distributed.

“Regeneration”. Regeneration refers to the development of a plant fromtissue culture.

“Resistance”. As used herein the terms “resistance” and “tolerance” (andgrammatical variations thereof) are used interchangeably to describeplants that show reduced or essentially no symptoms to a specific biotic(e.g., a pest, pathogen or disease) or abiotic (e.g., exogenous orenvironmental, including herbicides) factor or stressor. In someembodiments, “resistant” or “tolerant” plants show some symptoms but arestill able to produce marketable product with an acceptable yield, e.g.,the yield may still be reduced and/or the plants may be stunted ascompared with the yield or growth in the absence of the biotic and/orabiotic factor or stressor. Those skilled in the art will appreciatethat the degree of resistance or tolerance may be assessed with respectto a plurality or even an entire field of plants. A tomato plant may beconsidered “resistant” or “tolerant” if resistance/tolerance is observedover a plurality of plants (e.g., an average), even if particularindividual plants may be susceptible to the biotic or abiotic factor orstressor.

“RHS”. RHS refers to the Royal Horticultural Society of England whichpublishes an official botanical color chart quantitatively identifyingcolors according to a defined numbering system. The chart may bepurchased from Royal Horticulture Society Enterprise Ltd., RHS Garden;Wisley, Woking; Surrey GU236QB, UK.

“Single locus conversation”. A single locus converted or conversionplant refers to a plant that is developed by plant breeding techniques(e.g., backcrossing), genome editing techniques, genetic transformationtechniques and/or mutation techniques wherein essentially all of thedesired morphological and physiological characteristics of a line arerecovered in addition to the single locus introduced into the line viathe plant breeding, genome editing, genetic transformation, or mutationtechniques.

“Substantially equivalent characteristic”. A characteristic that, whencompared, does not show a statistically significant difference (e.g.,p=0.05) from the mean.

“Transgene”. A nucleic acid of interest that can be introduced into thegenome of a plant by genetic engineering techniques (e.g.,transformation) or breeding. The transgene can be from the same or adifferent species. If from the same species, the transgene can be anadditional copy of a native coding sequence or can present the nativesequence in a form or context (e.g., different genomic location and/orin operable association with exogenous regulatory elements such as apromoter) than is found in the native state. The transgene can comprisean open reading frame encoding a polypeptide or can encode a functionalnon-translated RNA (e.g., an interfering RNA [RNAi]).

Botanical Description of Tomato Line AI1D13TEN000015 and Hybrid T316108.

When grown under standard horticultural conditions (e.g., in agreenhouse), the mature fruit of hybrid tomato T316108 is characterizedby a number of traits including, without limitation: a mature fruithaving a shiny pink skin color and flesh, oval plum shape, an averageweight of about 10 grams, and an excellent taste.

When grown under standard horticultural conditions (e.g. in agreenhouse), the mature fruit of tomato line AI1D13TEN000015 ischaracterized by a number of traits including, without limitation: amature fruit having a yellow skin color, a uniform yellow fruit flesh,oval plum shape, an average weight of about 9 to 10 grams, and anaverage diameter of about 2.8 cm.

Tomato line AI1D13TEN000015 has shown uniformity and stability withinthe limits of environmental influence. It has been self-pollinated fornumerous generations with careful attention to uniformity of plant type.The variety has been increased with continued observation foruniformity. No variant traits have been observed or are expected intomato line AI1D13TEN000015.

More detailed botanical descriptions of hybrid T316108 and lineAI1D13TEN000015 are shown in Table 1 below.

TABLE 1 Variety Description Information (based on evaluation in apassive greenhouse). Comparison of hybrid T316108 with a similarvariety. Characteristic in State of expression of Denomination of whichthe similar State of expression candidate variety similar varietyvariety is different of similar variety (T316108) Sweetelle Cluster Longopen Shorter shape clusters compact clusters Sweetelle Plant Opengenerative Vigorous vegetation plant type vegetative plant typeSweetelle Fruit color Red Pink Additional information for tomato hybridT316108. Characteristic T316108 Plant: growth type Indeterminate Time ofmaturity Early-medium Level of setting Medium Seedling: anthocyanincoloration of hypocotyl Present Stem: anthocyanin coloration of upperthird Very strong Plant: height Medium Vigor of the plant Medium-strongLeaf: type of blade Bipinnate Leaf: intensity of green color MediumPeduncle: abscission layer Present (jointed) Fruit: size Verysmall-small Fruit: average weight in grams 10 Fruit: shape inlongitudinal section Obovate Fruit: number of locules Two or threeFruit: green shoulder (before maturity) Absent Fruit: green stripes(before maturity) Absent Fruit: color at maturity Pink Fruit: color offlesh (at maturity) Pink Fruit: glossiness of skin Shiny Fruit: firmnessMedium-Firm Fruit: ribbing at peduncle end Weak Fruit: shelf lifeMedium - long Fruit: shelf life in days 16 Homogeneity of size of thefruits Uniform fruit size Long shelf life gene(s) present? Absent Timeof maturity (±comparable with the Early-medium variety Sweetelle)Resistances: Fulvia fulva group A Susceptible Fulvia fulva group BSusceptible Fulvia fulva group C Susceptible Fulvia fulva group DSusceptible Fulvia fulva group E Susceptible Fusarium oxysporum f. sp.lycopersici race 0 Resistant Fusarium oxysporum f. sp. lycopersici race1 Susceptible Fusarium oxysporum f. sp. radicis-lycopersici Intermediateresistant Verticillium dahliae Susceptible Meloidogyne arenariaIntermediate resistant Meloidogyne incognita Intermediate resistantMeloidogyne javanica Intermediate resistant Tobacco Mosaic Virus race 0Highly resistant Tomato Mosaic Virus strain 0 Highly resistant TomatoMosaic Virus strain 1 Highly resistant Tomato Mosaic Virus strain 1-2Highly resistant Tomato Mosaic Virus strain 2 Highly resistant TomatoSpotted Wilt Virus Susceptible Tomato Yellow Leaf Curl Virus Not testedSensitivity to silvering Not tested Additional information for tomatoinbread line, AI1D13TEN000015. Characteristic AI1D13TEN000015 Plant:growth type Indeterminate Time of flowering Medium late Time of maturityMedium early Seedling: anthocyanin coloration of hypocotyl Present Stem:pubescence (hairiness) Sparsely hairy (scattered long hairs) Stem:branching Intermediate Stem: branching at cotyledonary or 1^(st) leafyAbsent node Stem: number of nodes between first Several (5-9)inflorescence Stem: number of nodes between early Few (1-4)inflorescences (1^(st)-2^(nd), 2^(nd)-3^(rd)) Stem: number of nodesbetween later Few (1-4) developing inflorescences Plant: height 180 cmVigor of the plant Medium-strong Leaf: type of blade Bipinnate Leaflet:margins Deeply toothed/cut toward base Leaflet: marginal rolling orwiltiness Strong Leaflet: onset of leaflet rolling Mid-season Leaflet:surface of major leaflets Rugose (bumpy/veiny) Leaf: pubescence NormalInflorescence: type (2^(nd) and 3^(rd) truss) Simple Leafy or “running”inflorescences Occasional Average number of flowers in inflorescence 18Flower: fasciation (1^(st) flower of inflorescence) Absent Flower:Pubescence of style Absent Flower: color Yellow Flower: anthers Allfused in tube Flower: calyx Normal, lobes awl-shaped Flower: calyx-lobesShorter than corolla Time of flowering (greenhouse) in no. of days 60after sowing Peduncle: abscission layer Present (jointed) Peduncle:length (from abscission layer to   8 mm calyx) Fruit: size Verysmall-small Fruit: size (average weight of 10 fruits) (g) Very small(<100) Fruit: average weight in grams 11 Fruit: typical shape Plum oregg shape Fruit length (cm)   2.5 Fruit: diameter (cm)   2.8 Fruit:shape in longitudinal section Elliptic Fruit: cross section Round Fruit:depression at peduncle end Indented Fruit: size peduncle scar SmallFruit: shape at blossom end Flat Fruit: size of core (in cross-section)Absent Fruit: thickness of pericarp Thin Fruit: epidermis Normal Fruit:epidermis color Colorless Fruit: epidermis texture Tender Fruit: numberof locules Two to three Fruit: green shoulder (before maturity) PresentFruit: intensity of green color of shoulder Light Fruit: green stripes(before maturity) Present Fruit: intensity of green color beforematurity Light Fruit: color at maturity Yellow Fruit: color of flesh (atmaturity) Uniform yellow Fruit: surface Smooth Fruit: locular gel colorof table ripe fruit Yellow Fruit: point of detachment at harvest Atcalyx attachment Fruit: shelf life Medium Homogeneity of size of thefruits Homogeneous Long shelf life gene(s) present? Absent Fruit: shapeof the pistil scar Dot Shape of the fruit in transverse section RoundResistances: Fulvia fulva group A Susceptible Fulvia fulva group BSusceptible Fulvia fulva group C Susceptible Fulvia fulva group DSusceptible Fulvia fulva group E Susceptible Fusarium oxysporum f. sp.lycopersici race 0 Susceptible Fusarium oxysporum f. sp. lycopersicirace 1 Susceptible Fusarium oxysporum f. sp. radices-lycopersiciSusceptible Verticillium albo-atrum Susceptible Verticillium dahliaSusceptible Meloidogyne arenaria Intermediately resistant Meloidogyneincognita Intermediately resistant Meloidogyne javanica Intermediatelyresistant Tobacco Mosaic Virus race 0 Highly resistant Tomato MosaicVirus strain 0 Highly resistant Tomato Mosaic Virus strain 1 Highlyresistant Tomato Mosaic Virus strain 1-2 Highly resistant Tomato MosaicVirus strain 2 Highly resistant Tomato Spotted Wilt Virus SusceptibleTomato Yellow Leaf Curl Virus Susceptible Sensitivity to silvering NottestedTissue Culture.

In embodiments, tomato plants can be propagated by tissue culture andregeneration. Tissue culture of various plant tissues and regenerationof plants therefrom is well known. For example, reference may be had toTeng, et al., HortScience, 27:9, 1030-1032 (1992); Teng, et al.,HortScience, 28:6, 669-1671 (1993); Zhang, et al., Journal of Geneticsand Breeding, 46:3, 287-290 (1992); Webb, et al., Plant Cell Tissue andOrgan Culture, 38:1, 77-79 (1994); Curtis, et al., Journal ofExperimental Botany, 45:279, 1441-1449 (1994); Nagata, et al., Journalfor the American Society for Horticultural Science, 125:6, 669-672(2000); and Ibrahim, et al., Plant Cell Tissue and Organ Culture, 28(2),139-145 (1992). It is clear from the literature that the state of theart is such that these methods of obtaining plants are routinely usedand have a high rate of success. Thus, another aspect of this inventionis to provide cells which upon growth and differentiation produce tomatoplants having desired characteristics of tomato hybrid T316108 or lineAI1D13TEN000015. Optionally, tomato plants can be regenerated from thetissue culture of the invention comprising all or essentially all of thephysiological and morphological characteristics of tomato hybrid T316108or line AI1D13TEN000015.

As used herein, the term “tissue culture” indicates a compositioncomprising isolated cells of the same or a different type or acollection of such cells organized into parts of a plant. Exemplarytypes of tissue cultures are protoplasts, calli, meristematic cells, andplant cells that can generate tissue culture that are intact in plantsor parts of plants, such as leaves, pollen, embryos, roots, root tips,anthers, pistils, flowers, seeds, petioles, suckers, and the like. Meansfor preparing and maintaining plant tissue culture are well known in theart. By way of example, a tissue culture comprising organs has been usedto produce regenerated plants. U.S. Pat. Nos. 5,959,185, 5,973,234, and5,977,445 describe certain techniques.

Additional Breeding Methods.

This invention is also directed to methods for producing a tomato plantby crossing a first parent tomato plant with a second parent tomatoplant wherein the first and/or second parent tomato plant is a plant oftomato hybrid T316108 or line AI1D13TEN000015. Thus, any of thefollowing exemplary methods using tomato hybrid T316108 or lineAI1D13TEN000015 are part of this invention: selfing, backcrosses, hybridproduction, crosses to populations, double haploid production, and thelike. All plants produced using tomato hybrid T316108 or lineAI1D13TEN000015 as at least one parent are within the scope of thisinvention, including those developed from tomato plants derived fromtomato hybrid T316108 or line AI1D13TEN000015. Advantageously, tomatohybrid T316108 or line AI1D13TEN000015 can be used in crosses withother, different, tomato plants (e.g., inbred lines) to produce firstgeneration (F₁) tomato hybrid seeds and plants with desirablecharacteristics. The tomato plants of the invention can also be used fortransformation where exogenous transgenes are introduced and expressedby the plants of the invention or for introduction of genetic changes bygene editing or mutagenesis. Genetic variants created either throughtraditional breeding methods, gene editing, mutagenesis ortransformation of the cultivars of the invention by any of a number ofprotocols known to those of skill in the art are intended to be withinthe scope of this invention.

The following describes exemplary breeding methods that may be used withtomato hybrid T316108 or line AI1D13TEN000015 in the development offurther tomato plants. One such embodiment is a method for developingtomato hybrid T316108 or line AI1D13TEN000015 progeny tomato plants(e.g., a diploid progeny tomato plant) in a tomato plant breedingprogram comprising: obtaining a plant, or a part thereof, of tomatohybrid T316108 or line AI1D13TEN000015, utilizing said plant or plantpart as a source of breeding material, and selecting a tomato hybridT316108 or line AI1D13TEN000015 progeny plant with some, all oressentially all morphological and/or physiological characteristics oftomato hybrid T316108 or line AI1D13TEN000015 (see, e.g., Table 1),respectively, optionally with the use of molecular markers. Inrepresentative embodiments, a progeny plant of tomato hybrid T316108 hasat least 1, 2, 3, 4 or more of the morphological and physiologicalcharacteristics of tomato hybrid T316108 (for example, produces a maturefruit having a shiny pink skin color, a pink mature fruit flesh, ovalplum shape, an average weight of about 10 grams, and an excellenttaste). In embodiments, a progeny plant of tomato line AI1D13TEN000015has at least 1, 2, 3, 4 or more of the characteristics of tomato lineAI1D13TEN000015 (for example, produces a mature fruit having a yellowskin color, a uniform yellow fruit flesh, oval plum shape, an averageweight of about 9 to 10 grams, and an average diameter of about 2.8 cm).In embodiments, a progeny plant of tomato hybrid T316108 or tomato lineAI1D13TEN000015 comprises essentially all or even all of themorphological and physiological characteristics of tomato hybrid T316108or line AI1D13TEN000015, respectively so that said progeny tomato plantis not significantly different for said traits than tomato hybridT316108 or line AI1D13TEN000015, respectively, as determined at the 5%significance level when grown in the same environmental conditions;optionally, with the presence of one or more desired added traits.Breeding steps that may be used in the breeding program include pedigreebreeding, backcrossing, mutation breeding, recurrent selection and/ordouble haploid production. In conjunction with these steps, techniquessuch as RFLP-enhanced selection, genetic marker enhanced selection (forexample, SSR markers) and/or and the making of double haploids may beutilized.

Another representative method involves producing a population of tomatohybrid T316108 or line AI1D13TEN000015 progeny plants (e.g., diploidprogeny plants), comprising crossing tomato hybrid T316108 or lineAI1D13TEN000015 with another tomato plant, thereby producing apopulation of tomato plants that, on average, derives at least 6.25%,12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%,95%, 96%, 97%, 98% or 99% of its alleles (i.e., TAC) from tomato hybridT316108 or line AI1D13TEN000015, e.g., at least about 6.25%, 12.5%, 25%,30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%,97%, 98% or 99% of the genetic complement of tomato hybrid T316108 orline AI1D13TEN000015. One embodiment of this invention is the tomatoplant produced by this method and that has obtained at least 6.25%,12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%,95%, 96%, 97%, 98% or 99% of its alleles (i.e., TAC) from tomato hybridT316108 or line AI1D13TEN000015, and optionally may be the result of abreeding process comprising one or two breeding crosses and one or moreof selfing, sibbing, backcrossing and/or double haploid techniques inany combination and any order. In embodiments, the breeding process doesnot include a breeding cross, and comprises selfing, sibbing,backcrossing and or double haploid technology.

One of ordinary skill in the art of plant breeding would know how toevaluate the traits of two plants to determine if there is or is notsignificant difference between the two traits expressed by those plants.For example, see Fehr and Walt, Principles of Cultivar Development, pp.261-286 (1987). Thus, in embodiments, the invention includes tomatohybrid T316108 progeny tomato plants characterized by e.g., 1, 2, 3, 4or more characteristics of tomato hybrid T316108 as described herein,e.g., produces a mature fruit having a shiny pink skin color, a pinkmature fruit flesh, oval plum shape, an average weight of about 10grams, and an excellent taste. In embodiments, the invention includestomato line AI1D13TEN000015 progeny tomato plants characterized by 1, 2,3, 4 or more of the characteristics of tomato line AI1D13TEN000015 asdescribed herein, e.g., produces a mature fruit having a yellow skincolor, a uniform yellow fruit flesh, oval plum shape, an average weightof about 9 to 10 grams, and an average diameter of about 2.8 cm. Inembodiments, the selected progeny comprises all or essentially all themorphological and physiological characteristics of tomato hybrid T316108or line AI1D13TEN000015 (e.g., a described in Table 1). In embodiments,the invention encompasses progeny plants having a combination of atleast 2, 3, 4, 5, 6, 7, 8, 9, 10 or more of the characteristics asdescribed herein for tomato hybrid T316108 or line AI1D13TEN000015 (see,e.g., Table 1), so that said progeny tomato plant is not significantlydifferent for said traits than tomato hybrid T316108 or lineAI1D13TEN000015, respectively, as determined at the 5% significancelevel when grown in the same environmental conditions. Using techniquesdescribed herein and those known in the art, molecular markers may beused to identify said progeny plant as progeny of tomato hybrid T316108or line AI1D13TEN000015. Mean trait values may be used to determinewhether trait differences are significant, and optionally the traits aremeasured on plants grown under the same environmental conditions.

Progeny of tomato hybrid T316108 or line AI1D13TEN000015 may also becharacterized through their filial relationship with tomato hybridT316108 or line AI1D13TEN000015, as for example, being within a certainnumber of breeding crosses of tomato hybrid T316108 or lineAI1D13TEN000015. A breeding cross is a cross made to introduce newgenetics into the progeny, and is distinguished from a cross, such as aself or a sib cross or a backcross to either tomato hybrid T316108 orline AI1D13TEN000015 as a recurrent parent, made to select amongexisting genetic alleles. The lower the number of breeding crosses inthe pedigree, the closer the relationship between tomato hybrid T316108or line AI1D13TEN000015 and its progeny. For example, progeny producedby the methods described herein may be within 1, 2, 3, 4, 5 or morebreeding crosses of tomato hybrid T316108 or line AI1D13TEN000015. Thoseskilled in the art will appreciate that a progeny of hybrid T316108 isalso a progeny of parental line AI1D13TEN000015, just the generationalrelationship is different.

In representative embodiments, a tomato plant derived from tomato hybridT316108 or line AI1D13TEN000015 comprises cells comprising at least oneset of chromosomes derived from tomato hybrid T316108 or lineAI1D13TEN000015. In embodiments, the tomato plant or population oftomato plants derived from tomato hybrid T316108 or line AI1D13TEN000015comprises, on average, at least 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%,50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of itsalleles (i.e., TAC) from tomato hybrid T316108 or line AI1D13TEN000015,e.g., at least about 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%,60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the geneticcomplement of tomato hybrid T316108 or line AI1D13TEN000015, andoptionally may be the result of one or more of selfing, sibbing,backcrossing and/or double haploid techniques in any combination. Inembodiments, the tomato plant derived from tomato hybrid T316108 or lineAI1D13TEN000015 is one, two, three, four, five or more breeding crossesremoved from tomato hybrid T316108 or line AI1D13TEN000015.

In representative embodiments, a plant derived from tomato hybridT316108 or line AI1D13TEN000015 is a double haploid plant, a hybridplant, an inbred plant, and/or a diploid plant.

In embodiments, a derived plant from tomato hybrid T316108 or lineAI1D13TEN000015 comprises a desired added trait. In representativeembodiments, a tomato plant derived from tomato hybrid T316108 or lineAI1D13TEN000015 comprises all of the morphological and physiologicalcharacteristics of tomato hybrid T316108 or line AI1D13TEN000015 (e.g.,as described in Table 1). In embodiments, the tomato plant derived fromtomato hybrid T316108 or line AI1D13TEN000015 comprises essentially allof the morphological and physiological characteristics of tomato hybridT316108 or line AI1D13TEN000015 (e.g., as described in Table 1), withthe addition of a desired added trait.

Those skilled in the art will appreciate that traits can be introducedinto tomato hybrid T316108 or line AI1D13TEN000015 by any method knownin the art, e.g., plant transformation methods, conventional breeding,gene editing and/or natural or induced mutations.

Further Embodiments of the Invention

With the advent of molecular biological techniques that have allowed theisolation and characterization of genes that encode specific proteinproducts, scientists in the field of plant biology developed a stronginterest in engineering the genome of plants to contain and expressforeign nucleic acids including additional or modified versions ofnative (endogenous) nucleic acids (optionally driven by a non-nativepromoter) in order to alter the traits of a plant in a specific manner.Any nucleic acid sequences, whether from a different species, the samespecies or an artificial sequence, which are introduced into the genomeusing transformation or various breeding methods, are referred to hereincollectively as “transgenes.” Over the last fifteen to twenty years,several methods for producing transgenic plants have been developed, andin particular embodiments the present invention also relates totransformed versions of tomato plants disclosed herein.

Genetic engineering techniques can be used (alone or in combination withbreeding methods) to introduce one or more desired added traits intoplant, for example, tomato hybrid T316108 or line AI1D13TEN000015 orprogeny or tomato plants derived thereof. Once a transgene has beenintroduction into a plant by genetic transformation, it can betransferred to other plants via conventional breeding.

Plant transformation generally involves the construction of anexpression vector that will function in plant cells. Optionally, such avector comprises one or more nucleic acids comprising a coding sequencefor a polypeptide or an untranslated functional RNA under control of, oroperatively linked to, a regulatory element (for example, a promoter).In representative embodiments, the vector(s) may be in the form of aplasmid, and can be used alone or in combination with other plasmids, toprovide transformed tomato plants using transformation methods asdescribed herein to incorporate transgenes into the genetic material ofthe tomato plant.

Additional methods include, but are not limited to, expression vectorsintroduced into plant tissues using a direct nucleic acid transfermethod, such as microprojectile-mediated delivery (e.g., with abiolistic device), DNA injection, Agrobacterium-mediated transformation,electroporation, and the like. Transformed plants obtained from theplants (and parts and tissue culture thereof) of the invention areintended to be within the scope of this invention.

Expression Vectors for Plant Transformation—Selectable Markers.

Expression vectors typically include at least one nucleic acidcomprising or encoding a selectable marker, operably linked to aregulatory element (for example, a promoter) that allows transformedcells containing the marker to be either recovered by negativeselection, e.g., inhibiting growth of cells that do not contain theselectable marker, or by positive selection, e.g., screening for theproduct encoded by the selectable marker. Many commonly used selectablemarkers for plant transformation are well known in the transformationart, and include, for example, nucleic acids that code for enzymes thatmetabolically detoxify a selective chemical agent which may be anantibiotic or an herbicide, or nucleic acids that encode an alteredtarget which is insensitive to the inhibitor. Positive selection methodsare also known in the art.

Commonly used selectable markers in plants include, but are not limitedto: neomycin phosphotransferase II (nptII) conferring resistance tokanamycin, hygromycin phosphotransferase conferring resistance to theantibiotic hygromycin, bacterial selectable markers that conferresistance to antibiotics (e.g., gentamycin acetyl transferase,streptomycin phosphotransferase, and aminoglycoside-3′-adenyltransferase, selectable markers conferring resistance to herbicides(e.g., glyphosate, glufosinate, or bromoxynil). Selection of transformedplant cells can also be based on screening presumptively transformedplant cells rather than direct genetic selection of transformed cellsfor resistance to a toxic substance such as an antibiotic; such markersinclude without limitation alpha-glucuronidase (GUS),alpha-galactosidase, luciferase, and Green Fluorescent Protein (GFP) andmutant GFPs.

Expression Vectors for Plant Transformation—Promoters.

Transgenes included in expression vectors are generally driven by anucleotide sequence comprising a regulatory element (for example, apromoter). Numerous types of promoters are well known in thetransformation arts, as are other regulatory elements that can be usedalone or in combination with promoters.

As used herein, “promoter” includes reference to a region of DNAupstream from the start of transcription and involved in recognition andbinding of RNA polymerase and other proteins to initiate transcription.A “plant promoter” is a promoter capable of initiating transcription inplant cells.

Examples of promoters under developmental control include promoters thatpreferentially initiate transcription in certain tissues, such asleaves, roots, seeds, fibers, xylem vessels, tracheids, or sclerenchyma.Such promoters are referred to as “tissue-preferred.” Promoters thatinitiate transcription only in certain tissue are referred to as“tissue-specific.” A “cell type” specific promoter preferentially drivesexpression in certain cell types in one or more organs, for example,vascular cells in roots or leaves. An “inducible” promoter is a promoterthat is under environmental control. Examples of environmentalconditions that may affect transcription by inducible promoters includeanaerobic conditions or the presence of light. Tissue-specific,tissue-preferred, cell type specific, and inducible promoters constitutethe class of “non-constitutive” promoters. A “constitutive” promoter isa promoter that is active under most environmental conditions.

Many suitable promoters are known in the art and can be selected andused to achieve the desired outcome.

Signal Sequences for Targeting Proteins to Subcellular Compartments.

Transport of polypeptides produced by transgenes to a subcellularcompartment such as the chloroplast, vacuole, peroxisome, glyoxysome,cell wall, or mitochondrion, or for secretion into the apoplast, isgenerally accomplished by means of operably linking a nucleotidesequence encoding a signal sequence to the 5′ and/or 3′ region of anucleic acid encoding the polypeptide of interest. Signal sequences atthe 5′ and/or 3′ end of the coding sequence target the polypeptide toparticular subcellular compartments.

The presence of a signal sequence can direct a polypeptide to either anintracellular organelle or subcellular compartment or for secretion tothe apoplast. Many signal sequences are known in the art. See, forexample, Becker, et al., Plant Mol. Biol., 20:49 (1992); Close, P. S.,Master's Thesis, Iowa State University (1993); Knox, C., et al.,“Structure and Organization of Two Divergent Alpha-Amylase Genes fromBarley,” Plant Mol. Biol., 9:3-17 (1987); Lerner, et al., PlantPhysiol., 91:124-129 (1989); Fontes, et al., Plant Cell, 3:483-496(1991); Matsuoka, et al., PNAS, 88:834 (1991); Gould, et al., J. Cell.Biol., 108:1657 (1989); Creissen, et al., Plant J, 2:129 (1991);Kalderon, et al., A short amino acid sequence able to specify nuclearlocation, Cell, 39:499-509 (1984); and Steifel, et al., Expression of amaize cell wall hydroxyproline-rich glycoprotein gene in early leaf androot vascular differentiation, Plant Cell, 2:785-793 (1990).

Foreign Polypeptide Transgenes and Agronomic Transgenes.

With transgenic plants according to the present invention, a foreignprotein can be produced in commercial quantities. Thus, techniques forthe selection and propagation of transformed plants, which are wellunderstood in the art, yield a plurality of transgenic plants which areharvested in a conventional manner, and a foreign polypeptide then canbe extracted from a tissue of interest or from total biomass. Proteinextraction from plant biomass can be accomplished by known methods whichare discussed, for example, by Heney and Orr, Anal. Biochem., 114:92-6(1981). According to a representative embodiment, the transgenic plantprovided for commercial production of foreign protein is a tomato plantof the invention. In another embodiment, the biomass of interest is seedand/or fruit.

Likewise, by means of the present invention, agronomic transgenes andother desired added traits can be expressed in transformed plants (andtheir progeny, e.g., produced by breeding methods). More particularly,plants can be genetically engineered to express various phenotypes ofagronomic interest or other desired added traits. Exemplary nucleicacids of interest in this regard conferring a desired added trait(s)include, but are not limited to, those transgenes that confer resistanceto confer resistance to plant pests (e.g., nematode or insect) ordisease (e.g., fungal, bacterial or viral), transgenes that conferherbicide tolerance, transgenes that confer male sterility, andtransgenes that confer or contribute to a value-added trait such asincreased nutrient content (e.g., iron, nitrate), increased sweetness(e.g., by introducing a transgene coding for monellin), modified fattyacid metabolism (for example, by introducing into a plant an antisensesequence directed against stearyl-ACP desaturase to increase stearicacid content of the plant), modified carbohydrate composition (e.g., byintroducing into plants a transgene coding for an enzyme that alters thebranching pattern of starch), modified fruit color (e.g., external fruitcolor and/or fruit flesh), or modified flavor profile of the fruit.

In embodiments, the transgene encodes a non-translated RNA (e.g., RNAi)that is expressed to produce targeted inhibition of gene expression,thereby conferring the desired trait on the plant.

In embodiments, the transgene encodes the machinery used for geneediting techniques.

Any transgene, including those exemplified above, can be introduced intothe tomato plants of the invention through a variety of means including,but not limited to, transformation (e.g., genetic engineeringtechniques), conventional breeding, and introgression methods tointroduce the transgene into other genetic backgrounds.

Methods for Plant Transformation.

Numerous methods for plant transformation have been developed, includingbiological and physical plant transformation protocols. See, forexample, Miki, et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, Glickand Thompson Eds., CRC Press, Inc., Boca Raton, pp. 67-88 (1993). Inaddition, expression vectors and in vitro culture methods for plant cellor tissue transformation and regeneration of plants are available. See,for example, Gruber, et al., “Vectors for Plant Transformation” inMethods in Plant Molecular Biology and Biotechnology, Glick and ThompsonEds., CRC Press, Inc., Boca Raton, pp. 89-119 (1993). Commonly usedplant transformation methods include Agrobacterium-mediatedtransformation and direct transgene transfer methods (e.g.,microprojectile-mediated transformation, sonication, liposome orspheroplast fusion, and electroporation of protoplasts or whole cells).

Following transformation of plant target tissues, expression ofselectable marker transgenes (e.g., as described above) allows forpreferential selection of transformed cells, tissues and/or plants,using regeneration and selection methods now well known in the art.

The foregoing methods for transformation are typically used to produce atransgenic tomato line. The transgenic tomato line can then be crossedwith another (non-transgenic or transgenic) line in order to produce anew transgenic tomato line. Alternatively, a transgene that has beenengineered into a particular plant using transformation techniques canbe introduced into another plant or line using traditional breeding(e.g., backcrossing) techniques that are well known in the plantbreeding arts. For example, a backcrossing approach can be used to movean engineered transgene from a public, non-elite inbred line into anelite inbred line, or from an inbred line containing a foreign transgenein its genome into an inbred line or lines which do not contain thattransgene. As used herein, “crossing” can refer to a simple X by Ycross, or the process of backcrossing, depending on the context.

Locus Conversions.

When the term “plant” is used in the context of the present invention,this term also includes any locus conversions of that plant or variety.The term “locus converted plant” as used herein refers to those plantsthat are developed, for example, by backcrossing, genome editing,genetic transformation and/or mutation, wherein essentially all of thedesired morphological and physiological characteristics of a variety(e.g., for tomato hybrid T316108, a mature fruit having a shiny pinkcolor, a pink mature fruit flesh, oval plum shape, an average weight ofabout 10 grams or any other trait described herein, e.g., in Table 1;and for tomato line AI1D13TEN000015, a mature fruit having a yellow skincolor, a uniform yellow fruit flesh, oval plum shape, an average weightof about 9 to 10 grams or any other trait described herein, e.g., inTable 1) are recovered in addition to the one or more genes introducedinto the variety.

To illustrate, backcrossing methods can be used with the presentinvention to improve or introduce a characteristic into the variety. Theterm “backcrossing” as used herein refers to the repeated crossing of ahybrid progeny back to the recurrent parent, e.g., backcrossing 1, 2, 3,4, 5, 6, 7, 8, 9, or more times to the recurrent parent. The parentalplant that contributes the gene for the desired characteristic is termedthe “nonrecurrent” or “donor parent.” This terminology refers to thefact that the nonrecurrent parent is generally used one time in thebreeding e.g., backcross) protocol and therefore does not recur. Thegene that is transferred can be a native gene, a mutated native gene ora transgene introduced by genetic engineering techniques into the plant(or ancestor thereof). The parental plant into which the gene(s) fromthe nonrecurrent parent are transferred is known as the “recurrent”parent as it is used for multiple rounds in the backcrossing protocol.Poehlman & Sleper (1994) and Fehr (1993). In a typical backcrossprotocol, the original variety of interest (recurrent parent) is crossedto a second variety (nonrecurrent parent) that carries the gene(s) ofinterest to be transferred. The resulting progeny from this cross arethen crossed again to the recurrent parent and the process is repeateduntil a plant is obtained wherein essentially all of the desiredmorphological and physiological characteristics of the recurrent parentare recovered in the converted plant in addition to the transferredgene(s) and associated trait(s) from the nonrecurrent parent.

Genetic Analysis of Tomato Hybrid T316108 or Line AI1D13TEN000015.

The invention further provides a method of determining a geneticcharacteristic of tomato hybrid T316108 or line AI1D13TEN000015 or aprogeny thereof, e.g., a method of determining a genotype of tomatohybrid T316108 or line AI1D13TEN000015 or a progeny thereof. Inembodiments, the method comprises detecting in the genome of a tomatohybrid T316108 or line AI1D13TEN000015 plant, or a progeny plantthereof, at least a first polymorphism (e.g., by detecting a nucleicacid marker by a method comprising nucleic acid amplification and/ornucleic acid sequencing). To illustrate, in embodiments, the methodcomprises obtaining a sample of nucleic acids from the plant anddetecting at least a first polymorphism in the nucleic acid sample.Optionally, the method may comprise detecting a plurality ofpolymorphisms (e.g., two or more, three or more, four or more, five ormore, six or more, eight or more or ten or more polymorphisms, etc.) inthe genome of the plant. In representative embodiments, the methodfurther comprises storing the results of the step of detecting thepolymorphism(s) on a computer readable medium. The invention furtherprovides a computer readable medium produced by such a method.

Although the present invention has been described with reference tospecific details of certain embodiments thereof, it is not intended thatsuch details should be regarded as limitations upon the scope of theclaimed invention except as and to the extent that they are included inthe accompanying claims.

DEPOSIT INFORMATION

Applicants have made a deposit of at least 2500 seeds of and tomatohybrid T316108 with American Type Culture Collection (ATCC), 10801University Boulevard, Manassas, Va., 20110-2209 U.S.A. under ATCCAccession No. PTA-126904 on Nov. 24, 2020 under the provisions of theBudapest Treaty. This deposit of tomato hybrid T316108 will bemaintained in the ATCC depository, which is a public depository, for aperiod of 30 years, or 5 years after the most recent request, or for theeffective life of the patent, whichever is longer, and will be replacedif any of the deposited seed becomes nonviable during that period.Additionally, Applicants have satisfied all the requirements of 37C.F.R. §§ 1.801-1.809, including providing an indication of theviability of the samples. Access to these deposits will be madeavailable during the pendency of this application to the Commissionerupon request. All restrictions on the availability of the depositedmaterial from ATCC to the public will be irrevocably and withoutrestriction removed upon granting of the patent. Applicants impose norestrictions on the availability of the deposited material from ATCC;however, Applicants have no authority to waive any restrictions imposedby law on the transfer of biological material or its transportation incommerce. Applicants do not waive any infringement of its rights grantedunder this patent or under the Plant Variety Protection Act (7 USC §2321 et seq.).

The foregoing invention has been described in detail by way ofillustration and example for purposes of clarity and understanding.However, it will be apparent that certain changes and modifications suchas single locus modifications and mutations, somaclonal variants,variant individuals selected from large populations of the plants of theinstant cultivars and the like may be practiced within the scope of theinvention.

What is claimed is:
 1. A seed that produces tomato hybrid T316108, arepresentative sample of seed having been deposited under ATCC AccessionNo. PTA-126904.
 2. A plant of tomato hybrid T316108 produced from theseed of claim
 1. 3. A tomato plant, or a part thereof, having all thephysiological and morphological characteristics of the tomato plant ofclaim
 2. 4. A seed that produces the plant of claim
 3. 5. A plant partof the plant of claim 2, wherein said plant part is a leaf, a fruit, ashoot, pollen, an ovule, an anther, a root, a rootstock, a scion, or acell.
 6. A tissue culture of regenerable cells of the tomato plant ofclaim
 2. 7. A tomato plant regenerated from the tissue culture of claim6 or a selfed progeny thereof, wherein said tomato plant comprises allof the physiological and morphological characteristics of tomato hybridT316108.
 8. A method of producing tomato seed, the method comprisingcrossing the plant of claim 2 with itself or a second tomato plant andharvesting the resulting seed.
 9. A grafted tomato plant comprising arootstock and a scion, wherein the plant of claim 2 is used as the scionand the rootstock is from a different tomato plant.
 10. A method ofproducing a grafted tomato plant, the method comprising: (a) providing ascion from the plant of claim 2; and (b) grafting the scion to arootstock from a different tomato plant.
 11. A method of developing atomato line in a tomato plant breeding program using plant breedingtechniques, which include employing a tomato plant, or a part thereof,as a source of plant breeding material, the method comprising: (a)obtaining the tomato plant, or part thereof, of claim 2 as a source ofbreeding material; and (b) applying plant breeding techniques to saidtomato plant or part thereof.
 12. A method for producing a seed of atomato plant derived from the tomato hybrid T316108, the methodcomprising: (a) crossing the plant of claim 2 with a different tomatoplant; (b) allowing seed to form; (c) growing a plant from the seed ofstep (b) to produce a plant derived from tomato hybrid T316108; (d)selfing the plant of step (c) or crossing it to a second tomato plant toform additional tomato seed derived from tomato hybrid T316108; and (e)optionally repeating steps (c) and (d) one or more times to generatefurther derived tomato seed from tomato hybrid T316108, wherein in step(c) a plant is grown from the additional tomato seed of step (d) inplace of growing a plant from the seed of step (b).
 13. A method ofvegetatively propagating tomato hybrid T316108, the method comprising:(a) collecting tissue capable of being propagated from the plant ofclaim 2; (b) cultivating the tissue to obtain proliferated shoots; (c)rooting the proliferated shoots to obtain rooted plantlets; and (d)optionally, growing plants from the rooted plantlets.
 14. A tomatoplantlet or plant obtained by the method of claim 13, wherein the tomatoplantlet or plant comprises all of the physiological and morphologicalcharacteristics of tomato hybrid T316108.
 15. A method of producing aplant of tomato hybrid T316108 comprising a desired added trait, themethod comprising introducing a transgene conferring the desired traitinto the plant of claim
 2. 16. A tomato plant produced by the method ofclaim 15, wherein the tomato plant comprises the transgene conferringthe desired trait and otherwise comprises all of the morphological andphysiological characteristics of tomato hybrid T316108.
 17. A seed thatproduces the plant of claim
 16. 18. A method for producing a tomatoseed, the method comprising selfing the plant of claim 2, which istomato hybrid T316108, for one or more generations and allowing seed toform.
 19. A method of producing a tomato fruit, the method comprising:(a) growing the tomato plant according to claim 2 to produce a tomatofruit; and (b) harvesting the tomato fruit.
 20. A method of producing aprocessed tomato product, the method comprising: (a) obtaining a fruitof the plant of claim 2; and (b) processing said fruit to produce aprocessed product.
 21. A method of determining a genotype of tomatohybrid T316108, the method comprising: (a) obtaining a sample of nucleicacids from the plant of claim 2; and (b) detecting a polymorphism in thenucleic acid sample using molecular biology techniques therebydetermining the genotype of tomato hybrid T316108.
 22. A plant of tomatohybrid T316108, or a seed that produces tomato hybrid T316108, said seeddeposited as ATCC Accession No. PTA-126904.